From the bench to onsite: turning discovery into technological solutions


Caparica, Portugal
Instituto Universitário Egas Moniz
Faculdade de Ciências e Tecnologia, Univ. NOVA de Lisboa

Peer-Reviewed Publications



C.M. Silveira, M.G. Almeida, J.J.G. Moura ”Nitrite biosensing- Electrochemical biosensors based on cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774”, Lambert Academic Publishing: 2014. ISBN 978-3-659-10593-7.


Book Chapters

Monteiro T., Zumpano R., Silveira C.M., Gabriela Almeida M. (2021) Selective Enzymes at the Core of Advanced Electroanalytical Tools: The Bloom of Biosensors. In: Moura J.J.G., Moura I., Maia L.B. (eds) Enzymes for Solving Humankind's Problems. Springer, Cham. pp 303-362

Monteiro T., Zumpano R., Silveira C.M., Gabriela Almeida M. (2021) Selective Enzymes at the Core of Advanced Electroanalytical Tools: The Bloom of Biosensors. In: Moura J.J.G., Moura I., Maia L.B. (eds) Enzymes for Solving Humankind's Problems. Springer, Cham. pp 303-362.

C. Correia, M. Rodrigues, C.M. Silveira, J.J.G. Moura, E. Ochoteco, E. Jubete, M.G. Almeida, “Nitrite Biosensing using Cytochrome c Nitrite Reductase: Towards a Disposable Strip Electrode”, in: J. Gabriel, J. Schier, S. Van Huffel, E. Conchon; C. Correia, A. Fred, H. Gamboa (Eds.): Biomedical Engineering Systems and Technologies, Communications in Computer and Information Science, 357, pp. 41-50. Springer, Heidelberg (2013). DOI:10.1007/978-3-642-38256-7_3

L.L. Gonçalves, M.G. Almeida, J. Lampreia, J.J.G. Moura, I. Moura (2005) “Prediction of Signal Peptides and Signal Anchors of Cytocrome c Nitrite Reductase from Desulfovibrio desulfuricans ATCC 27774 Using Bioinformatic Tools” in: D.S. Moss, S. Jelaska, S. Pongor editors, “Essays in Bioinformatics”. Vol. 368, p. 203-208, IOS Press, Amsterdam.



S. Besson, M.G. Almeida, C. Silveira (2022) ”Nitrite reduction in bacteria: a comprehensive view of nitrite reductases”, Coordination Chemistry Reviews, 464, 214560.

T. Monteiro, A.R. Coelho, M. Moreira, A.S. Viana, and M.G. Almeida (2021) Interfacing the enzyme multiheme cytochrome c nitrite reductase with pencil lead electrodes: towards a disposable biosensor for cyanide surveillance in the environment”, Biosensors and Bioelectronics191, 113438DOI: 10.1016/ j.bios .2021.113438


H. Lapa,  N. Silva,  A. Fantoni,  A.F. Maçarico,  G. AlmeidaE.C . B.A. Alegria, "Green synthesis of gold nanoparticles and their deposition on ITO surfaces", Proc. SPIE 11659, Colloidal Nanoparticles for Biomedical Applications XVI, 116590Q;  DOI: 10.1117/12.2582983


T. Monteiro, C. Dias, C.F. Lourenço, A. Ledo, R.M. Barbosa, and M.G. Almeida (2021) “Microelectrode Sensor for Real-Time Measurements of Nitrite in the Living Brain, in the Presence of Ascorbate”, Biosensors11, n.8. DOI: 10.3390/bios11080277


V.M. Moreira, P. Mascarenhas, V. Machado, J. Botelho, J.J. Mendes, N. Taveira, and M.G. Almeida (2021)”Diagnosis of SARS-Cov-2 infection using specimens other than naso- and oropharyngeal swabs: a systematic review and meta-analysis”, Diagnostics11, 363.

DOI: 10.3390/diagnostics11020363

C.M. Silveira, P.R. Rodrigues, W. Ghach, S.A. Pereira, M. Kranendonk, M. Etienne and M.G. Almeida (2021) ”Electrochemical activity of cytochrome P450 1A2 – the relevance of O2 control and the natural electron donor”, ChemElectroChem, in press, DOI: 10.1002/celc.202001255

V.M. Costa, J.P. Capela, J.R. Sousa, R.P. Eleutério, P.R.S. Rodrigues, J.L. Dores-Sousa, R.A. Carvalho, M.L. Bastos, J.A. Duarte, F. Remião,  M.G. Almeida, K.J. Varner, F. Carvalho (2020)  “Mitoxantrone impairs proteasome activity and prompts early energetic and proteomic changes in HL-1 cardiomyocytes at clinically relevant concentrations”, Archives of Toxicology,  94, 4067–4084. DOI: 10.1007/s00204-020-02874-4

C.M. Silveira, R. Zumpano, M. Moreira, M.P. Almeida, M.J. Oliveira, M. Bento, C. Montez, I. PaixãoR. Franco, E. Pereira, M.G. Almeida (2019) “Star‐Shaped Gold Nanoparticles as Friendly Interfaces for Protein Electrochemistry: the Case Study of Cytochrome c”, ChemElectroChem6(17), 4696-4703. DOI: 10.1002/celc.201901393

T. Monteiro, S. Gomes, E. Jubete, L. Añorga, C.M. Silveira, M.G. Almeida (2019) ”A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide”, Scientific Reports9, article n. 2622. DOI: 10.1038/s41598-019-39209-y

T. Monteiro, M.G. Almeida (2019) “Electrochemical enzyme biosensors revisited: old solutions for new problems”, Crit Rev Anal Chem., 49(1), 44-‌66, DOI:10.1080/10408347.2018.1461552

J.R. Sousa, C.M. Silveira, P. Fontes, C. Roma-Rodrigues, A.R. Fernandes, G. van Driessche, B. Devreese, I. Moura, J.J. Moura, M.G. Almeida (2017) “Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate- biosynthetic costs modulate substrate selection”, BBA – Proteins and Proteomics1865 (11),1455-1469. DOI: 10.1016/j.bbapap.2017.07.021

P. Branco, V. Kemsawasd, L. Santos, M. Diniz, J. Caldeira, M.G. Almeida, N. Arneborg, H. Albergaria, (2017) “Saccharomyces cerevisiae accumulates GAPDH-derived peptides on its cell surface that induce death of non-Saccharomyces yeasts by cell-to-cell contact”, FEMS Microbiology Ecology, 93(5), 1-10.  DOI: 10.1093/femsec/fix055

P. Branco, D. Francisco, M. Monteiro, M.G. Almeida, J. Caldeira, N. Arneborg, C. Prista, H. Albergaria (2017) “Antimicrobial properties and death-inducing mechanisms of saccharomycin, a biocide secreted by Saccharomyces cerevisiae”,  Applied Microbiol. Biotechnol.101(1), 159-171 DOI 10.1007/s00253-016-7755-6

C.M. Silveira, T. Monteiro, M.G. Almeida (2016) “Biosensing with paper-based miniaturized printed electrodes – a modern trend”, Biosensors, Biosensors, 6(4), 51.  DOI: 10.3390/bios6040051

T. Monteiro, F. Oliveira, A. Fins, C.G. Dias, C.M. Silveira, S.A. Pereira, M.G. Almeida (2016) “Assessment of human paraoxonase activity by electrochemistry: a simple and novel approach”, Anal. Methods, .8, 8141-8146.  DOI: 10.1039/C6AY01944G

H.A. Pedroso, C.M. Silveira, R.M. Almeida, A. Almeida, S. Besson, I. Moura, J.J.G. Moura, M.G. Almeida (2016) “Electron transfer and docking between cytochrome cd1 nitrite reductase and different redox partners – a comparative study”, BBA – Bioenergetics, 857(9), 1412-1421DOI: 10.1016/j.bbabio.2016.04.279

L. Santos, C. Silveira, E. Elamurugu, J.P. Neto, D. Nunes, L. Pereira, R. Martins, J. Viegas, J.J.G. Moura, S. Todorovic, M.G. Almeida, E. Fortunato (2016) “Synthesis of WO3 Nanoparticles for Biosensing Applications", Sensors Actuactors B 223, 186-194. DOI:10.1016/j.snb.2015.09.046 

V.M. Costa, J. Capela, C. Silveira, R.P. Eleutério, P. Rodrigues, M.L. Bastos, J. Duarte, F. Remião, M.G. Almeida, F. Carvalho (2015) “Mitoxantrone elicits oxidative stress and proteomic changes in HL-1 cardiomyocytes at clinically relevant doses” Toxicology Letters, 238(2), S267-S268. DOI:10.1016/j.toxlet.2015.08.771

V. Kemsawasd, P. Branco, M.G. Almeida, J. Caldeira, H. Albergaria, N. Arneborg (2015) “Cell-to-cell contact and antimicrobial peptides play a combined role in the death of Lachanchea thermotolerans during mixed-culture alcoholic fermentation with Saccharomyces cerevisiae”, FEMS Microbiol. Letters, 362(14). DOI:org/10.1093/femsle/fnv103

C.M. Silveira, P.O. Quintas, I. Moura, J.J.G. Moura, P. Hildebrandt, M.G. Almeida, S. Todorovic (2015) ”SERR spectroelectrochemical study of cytochrome cd1 nitrite reductase co-immobilized with physiological redox partner cytochrome c552 on biocompatible metal electrodes”. Plos One, 10(6): e0129940. DOI: 10.1371/journal.pone.0129940

T. Monteiro, P.R. Rodrigues, A.L. Gonçalves, J.J.G. Moura, L. Añorga, E. Jubete, B. Piknova, A.N. Schechter, C.M. Silveira, M.G. Almeida (2015) ”Construction of effective disposable biosensors for point-of-care testing of nitrite” Talanta, 142, 246–251. DOI: 10.1016/j.talanta.2015.04.057

R.R. Nair, C.M. Silveira, M.S. Dinis, M.G. Almeida, J.J.G. Moura, M.G. Rivas (2015) “Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by high molybdate levels”, J. Biol. Inorg. Chem., 20(2), 311-22. DOI: 10.1007/s00775-014-1224-4

P. Branco, D. Francisco, C. Chambon, M.l. Hébraud, N. Arneborg, M.G. Almeida, J. Caldeira, H. Albergaria (2014) “Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccharomyces cerevisiae and involved in wine microbial interactions”, Appl. Microbiol. Biotechnol. 98(2), 843-853. DOI: 10.1007/s00253-013-5411-y.

C.M. Silveira, M.G. Almeida (2013) ”Small electron-transfer proteins as mediators in enzymatic electrochemical biosensors” Anal. Bioanal. Chem., 405(11), 3619-3635. DOI:10.1007/s00216-013-6786-4.

C. Silveira, M. Pimpão, P. Rodrigues, H. Pedroso, F. Pereira, J.J.G. Moura, M.G. Almeida (2013) “Probing the surface chemistries of different oxidized MWCNTs for the improved electrical wiring of cytochrome c nitrite reductase” Electrochem Comm., 35, 17–21.  DOI:10.1016/j.elecom.2013.07.027.

A.S. Serra, S. Jorge, C. Silveira, J.J.G. Moura, E. Jubete, E. Ochoteco, M.G. Almeida (2011) ”Cooperative use of Cytochrome cd1 Nitrite Reductase and its Redox Partner Cytochrome c552 to Improve the Selectivity of Nitrite Biosensing” Anal. Chim. Acta, 693 (1-2), 41-46. DOI:10.1016/j.aca.2011.03.029.

M.G. Almeida, A.S. Serra, C. Silveira, J.J.G. Moura (2010) ”Nitrite Biosensing Via Selective Enzymes – A Long But Promising Route” Sensors, 10(12) 11530-11555; DOI:10.3390/s101211530.

C. Silveira, J. Baur, S. Cosnier, J.J.G. Moura, M. Holzinger, S. Cosnier, M.G. Almeida (2010) "Enhanced direct electron transfer of a multihemic nitrite reductase on SWCNT modified electrodes" Electroanalysis, 22 (24), 2973–2978. DOI: 10.1002/elan.201000363.

C. M. Silveira, S. Besson, I. Moura, J.J.G. Moura, M.G. Almeida (2010) ”Measuring the Cytochrome c Nitrite Reductase Activity – Practical considerations on the enzyme assays” Bioinorg. Chem. Applic., Volume 2010, Article ID 634597, 8 pages. DOI:10.1155/2010/634597.

C.M. Silveira, S.P. Gomes, A.N. Araújo, C.M.C.M. Couto, M.C.B.S.M. Montenegro, R. Silva, A.S. Viana, S. Todorovic, J.J.G. Moura, M.G. Almeida (2010) “An Efficient Mediatorless Biosensor for Nitrite Determination” Biosens. Bioelectron. 25, 2026-2032. DOI:10.1016/j.bios.2010.01.031.

Z. Zhang, S. Xia, D. Leonard, N. Jaffrezic-Renault, J. Zhang, F. Bessueille, Y.s Goepfert, X. Wang, L. Chen, Z. Zhu, J. Zhao, M.G. Almeida, C.M. Silveira (2009) “A novel nitrite biosensor based on conductometric electrode modified with cytochrome c nitrite reductase composite membrane” Biosens. Bioelectron. 24, 1574-1579. DOI:10.1016/j.bios.2008.08.010.

H. Chen, C. Mousty, S. Cosnier, C. Silveira, J.J.G. Moura, M.G. Almeida (2007) “Highly Sensitive ccNiR Biosensor Electrical-Wired by [ZnCrAQS2] for Nitrite Determination” Electrochem. Comm. 9, 2240-2245. DOI:10.1016/j.elecom.2007.05.030

S.P. Gomes, M. Odlozilíková, M.G. Almeida, A.N. Araújo, C.M.C.M. Couto, M.C.B.S.M. Montenegro (2007) “Application of Lactate Amperometric Sol-Gel Biosensor to Sequential Injection Determination of L-Lactate”,   J. Pharm. Biomed. Anal. 43(4), 1376-1381. DOI:10.1016/j.jpba.2006.11.027.

M.G. Almeida, C.M. Silveira, J.J.G. Moura (2007) “Biosensing Nitrite Using the System Nitrite Redutase/Nafion/Methyl Viologen - A Voltammetric Study”, Biosens. Bioelectron. 22(11), 2485-2492. DOI:10.1016/j.bios.2006.09.027.

M.G. Almeida, C.M. Silveira, B. Guigliarelli, P. Bertrand, J.J.G. Moura, I. Moura, C. Léger (2007) “A Needle in a Haystack: the Active Site of the Membrane-bound Complex Cytochrome c Nitrite Reductase” FEBS Lett. 581(2), 284-288. DOI:10.1016/j.febslet.2006.12.02.

S. da Silva, S. Cosnier, M.G. Almeida, J.J.G. Moura (2004)An Efficient Poly(pyrrole)-Nitrite Reductase Biosensor for the Mediated Detection of Nitrite” Electrochem. Comm.  6(4), 404-408. DOI:10.1016/j.elecom.2004.02.007.

M.G. Almeida (2004) “Biossensores: Modernas Ferramentas para Monitorização e Controlo Analítico”, Bol. Biotecnol. 79, 12-23.

C.A. Cunha, S. Macieira, J.M. Dias, G. Almeida, L.L. Gonçalves, C. Costa, J. Lampreia, R. Huber, J.J.G. Moura, I. Moura, M.J. Romão (2003) “Cytochrome c Nitrite Reductase from Desulfovibrio desulfuricans ATCC 27774. The Relevance of the Two Calcium Sites in the Structure of the Catalytic Subunit (NrfA)”  J. Biol. Chem. 278, 17455-17465. DOI:10.1074/jbc.M211777200

M.G. Almeida, S. Macieira, L.L. Gonçalves, R. Huber, C.A. Cunha, M.J. Romão, C. Costa, J. Lampreia, J.J.G. Moura, I. Moura (2003) “The Isolation and characterization of Cytochrome c Nitrite Reductase Subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774. Re-evaluation of the spectroscopic data and redox properties”, Eur. J. Biochem. 270, 3904-3915. DOI:10.1046/j.1432-1033.2003.03772.x

G. Almeida, C. Rodrigues, J. Lampreia (2001) “Proteómica: a Interface entre a Biologia Molecular e a Bioquímica de Proteínas”, Bol. Soc. Port. Química 82, 49-56.

M.G. Almeida, M. Humanes, R. Melo, J.A. Silva, J.J.R. Fraústo da Silva, R. Wever (2000) “Purification and Characterization of Vanadium Haloperoxidases from the Brown Alga Pelvetia canaliculata of the Portuguese Coast”, Phytochemistry 54(1), 5-11. DOI:10.1016/S0031-9422(99)00602-0

J.M. Dias, C.A. Cunha, G. Almeida, C. Costa, J. Lampreia, J.J.G. Moura, I. Moura, M.J. Romão (2000) “Crystallization and Preliminary X-Ray Analysis of a Membrane Bound Nitrite Reductase from Desulfovibrio desulfuricans 27774”, Acta Crystallographica D56, 215-217.DOI:10.1107/S0907444999016066

S. Girotti, E.N. Ferri, F. Fini, F. Ruffini, R. Budini, I. Mura, G. Almeida, C. Costa, J.J.G. Moura, G. Carrea (1999) “Enzymatic Spectrophotometric Determination of Nitrites in Beer”, Anal. Lett. 32(11), 2217-2227. DOI: 10.1080/00032719908542965.